Journal: Cancer research

Article Title: SIRT1 is essential for oncogenic signaling by estrogen/estrogen receptor ? in breast cancer

doi: 10.1158/0008-5472.CAN-11-1446

Figure Lengend Snippet: (A) SIRT1 was immunoprecipitated (IP) from ER− (HMEC, MCF10A, MB231 and MB453) and ER+ (MCF7 and ZR75.1) cells using SIRT1 or ERα antibodies. Immunoblotting (IB) was done with SIRT1 or ERα antibodies. (B) Colocalization of SIRT1 and ERα in MCF-7 and ZR75.1 cells. Hoechst staining was used to locate the nucleus. The scale bars represent 20 µm. (C) ER− MCF10A and MB231 cells were transfected with an expression construct of ERα. IP and IB were performed 48 h post-transfection with SIRT1 and ERα antibodies. (D) MCF10A cells were transfected with an ERα expression construct, and colocalization (arrows) was monitored with SIRT1 and ERα antibodies.

Article Snippet: Cell lines, plasmids and transfection The human normal mammary epithelial cell line HMEC was obtained from the Lonza, Walkersville, MD while the other two human normal mammary epithelial cell lines, MCF10A and MCF12A, were obtained from ATCC, Manassas, VA. HBL100, also a human normal mammary epithelial cell line, was kindly provided by Dr. S. Sukumar, Johns Hopkins University, Baltimore, MD.

Techniques: Immunoprecipitation, Western Blot, Staining, Transfection, Expressing, Construct

Journal: BMC Cancer

Article Title: Ras-association domain family 1C protein promotes breast cancer cell migration and attenuates apoptosis

doi: 10.1186/1471-2407-10-562

Figure Lengend Snippet: RASSF1A and RASSF1C mRNA expression in epithelial and breast cancer cell lines

Article Snippet: The human mammary epithelial cell line AG1132B was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Expressing